The vector (target) of our cloning, in this case, is our plasmid. At the top right, select EcoRI and PstI as your restriction enzymes. Now repeat the same process for the suffix and insert it at the end of the GFP sequence. Paste the sequence for the biobrick prefix in the textbox and click on Insert. Then click on Edit, go to Insert, and select Bases. Next, open the file containing the GFP sequence and click on the beginning of the sequence. To do this, copy the biobrick prefix from the plasmid using the Sequence view by clicking View and selecting Sequence. The next step is to insert some bases into the sequence of the GFP to introduce new restriction sites that do not cut within the GFP itself. The details of the plasmid can also be seen in the pane on the left side of the window. We should now be able to see the plasmid in the window as in the image below. We will start by importing the plasmid by clicking on File -> import -> SnapGene online sequence -> Select pSB1C3 in the popup and click on Import.
![snapgene viewer alternatives snapgene viewer alternatives](https://1.bp.blogspot.com/-cuAYIq4JrJY/WbIlc9nnSbI/AAAAAAAABOM/odCv0-X0s4cNkSI-59fQ9eQrYP6uDUY3QCLcBGAs/s640/SnapGene.png)
Let’s now create an example by inserting GFP into a plasmid with chloramphenicol resistance. Hovering your mouse over any of the enzymes will show its dual.
![snapgene viewer alternatives snapgene viewer alternatives](https://www.esoftner.com/wp-content/uploads/2020/02/SnapGene-Free-Download.png)
Unique cutters will highlight enzymes that cut the sequence in only one place, while unique and dual cutters will show unique enzymes that cut the plasmid in two places. To do that, click on choose enzyme set, which is the drop-down arrow next to the show enzymes button on the left, and click on unique cutters or unique and dual cutters. The first step in this process is to select an enzyme set based on your protocol.
#Snapgene viewer alternatives how to#
This post will show an example of how to use SnapGene for restriction cloning using one of the protocols available in SnapGene. The cut genes are then joined with a method known as ligation.
![snapgene viewer alternatives snapgene viewer alternatives](https://www.saashub.com/images/app/context_images/26/c7d3531791dc/dnastar-lasergene-alternatives-medium.png)
Restriction cloning is a method of editing genes by cutting them with restriction enzymes at restriction sites.